The Zandstra lab has a large (almost 10,000 square feet), modern laboratory located in the Terrence Donnelly Centre for Cellular and Biomolecular Research at the University of Toronto (St George campus). The lab is located on the 11th floor and is shared with two other labs (van der Kooy and Audet labs) both focus on stem cell biology and bioengineering. The lab is run as an open concept, interdisciplinary, shared resource facility. It is equipped with state-of-the-art cell and molecular biology equipment including: cell type specific tissue culturing facilities, real-time PCR machines, protein and cell imaging systems. Specialized equipment includes: state-of-the-art microscopes, oxygen controlled incubators, two analytical flow cytometers, flow sorter, automated high-content screening microscopes, micro-fabrication and 3D printing equipment, bioreactor used for large-scale production of cells includes a 8-vessel Dasgip stirred tank bioreactor system at scales of 35-2000mL (Eppendorf), the Wave/Xuri rocking bag system (GE), our novel fed-batch bag system for blood cell culture, the MRT-24 microreactor system (Pall), and the Ambr-24 controlled small scale system (Sartorius), as well as various uncontrolled spinner flask and orbital shaker systems. Computational resources in the Zandstra lab include a heterogeneous network of Linux- and Windows-based computers and workstations. Machines range from desktops containing consumer-ready components to workstations configured for coding high performance and GPU-computing solutions.